We will write a custom essay sample on bacterial transformation lab report specifically the +pglo with lb/amp/ara has glowing bacteria due to the transformation. Restriction enzyme digestion lab, page 1 unfortunately, there was a mix-up during the preparation for this week's lab two different plasmids, pglo and pblu. Transformation lab report introduction transformation is the transfers of virulence from one cell to another, through the transferring of genetic material it was originally postulated in 1928 through the works of federick griffith, a british microbiologist. The original bacteria and the pglo plasmids dna can be eliminated since no fluorescence colour was observed under the uv light.
Genetic transformation of bacteria with the gene for green fluorescent protein (gfp) lab bac3 adapted from biotechnology explorer pglo™ bacterial transformation kit. Pglo transformation lab (ap lab 7) bio-rad lab book ori pglo bla gfp arac. A lab extension of the pglo lab may involve modifying more complex organisms than bacteria for example, one could attempt to influence the expression of the gfp gene in fish, or another multi cellular organism. In this lab, you will be using non-pathogenic e coli bacteria and pglo, a plasmid modified with two genes the the pglo plasmid contains the genetic codes for (see table 2).
Paul andersen explains the two major portions of the molecular biology lab in ap biology he starts by discussing the process of transformation he explains how you can use the pglo plasmid to produce glowing e coli bacteria. Ap bio pglo transformation formal lab report essay sample abstract: the topic of this research involved the occurrence of genetic transformation in bacteria (e coli. By comparing the +pglo plates with the control or the -pglo plates, it was easy to identify the traits which were not transformed traits that were originally observed which did not seem to become altered include. In the pglo bacterial transformation kit, students transform bacteria with a gene from a bioluminescent jellyfish to express a new protein, green fluorescent protein (gfp) they can turn the genes on and off to study gene regulation.
Materials for each lab group gloves safety goggles if desired waterproof marker microtube rack 6 disposable pipettes 6 disposable inoculating loops. You will now observe a transformation experiment using the pglo plasmid in addition to the gfp and ampicillin resistance genes, the bio-rad pglo plasmid contains several genes that control the expression of gfp. The +pglo plates can be compared to the -pglo plates again, pglo offers ampicillin resistance, so technically, all bacteria on the +pglo plates should have been transformed additionally, to determine if all bacteria on the +pglo plates were transformed, look at the +pglo plate with arabinose.
Learn pglo lab with free interactive flashcards choose from 249 different sets of pglo lab flashcards on quizlet. Information you will need for this week's pglo transformation lab, where you will transform e coli cells. Plasmid dna isolation and restriction enzyme digests record all procedures and data in your lab notebook, indicating who performed a specific procedure.
© copyright, cold spring harbor laboratoryall rights reserved. The pglo plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms the plasmid contains several reporter genes,. Paul andersen explains the two major portions of the molecular biology lab in ap biology he starts by discussing the process of transformation he explains how you can use the pglo plasmid to.
Start studying pglo learn vocabulary, terms, and more with flashcards, games, and other study tools. Transformation pvib lab answers introduction the transformation of bacterial cells is a useful experiment to help develop an understanding of transformation by plasmid dna. View lab report - pglo lab key from science anatomy at lambert high school appendix teacher's answer guide lesson 1 focus questions 1 to geneticaﬂy transform an entire organism, you must insert.
Bacterial transformation the pglo in this lab, your students will perform a procedure known as a genetic transformation. Title: pglo transformation lab introduction: genetic transformation is a change caused by genes, involving the insertion of a gene into an organism to change the organism¶s trait in this experiment, bacteria will be transformed with a gene that codes for green fluorescent protein (gfp) the. Lab 16 - rapid colony the ampicillin resistance gene (carried on the recombinant pglo plasmid we are using) lab 16 - bacterial transformation. Pglo transformation lab answer keypdf free pdf download now source #2: pglo transformation lab answer keypdf free pdf download 26,600 results any time.